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Intermediate

Part:BBa_K539392:Design

Designed by: Shu-Han Chang   Group: iGEM11_NCTU_Formosa   (2011-09-27)

Vio operon ABDE(each preceded by their own ribosome binding site) with a terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1029
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3814
    Illegal NgoMIV site found at 6040
    Illegal AgeI site found at 4010
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 5239
    Illegal SapI.rc site found at 5314


Design Notes

In front of this parts ,you can apply to your design.

We have designed the primer to correct the point mutation of XbaI site already.

Please refer to BBa_K274004 Experience

Source

vioABDE(Part:BBa_K274004) is taken from 2008 Cambridge iGEM

References